Battle Moveset

A Good Moveset for Jumpluff would have to be this:

Attacks:

Encore/Hidden Power [Flying]
Leech Seed
Sleep Powder
Substitute

Items Attached:

LeftOvers

Preferred Nature:

Jolly

Strategy Using Jumpluff

Jumpluff can be one of the most frustrating Pokémon to face, both in OU and UU battles. With a huge speed stat it can use Sleep Powder to put to sleep an opponent's Pokémon and then force a switch to set up Leech Seed. From there your opponent will simply use Substitute repeatedly until you switch, and then repeat to set up Leech Seed.

The choice between an offensive move and Encore is a matter of personal preference. With Hidden Power [Flying] (or Aerial Ace for those unable to obtain it) you can KO Grass typed Pokémon (who aren't effected by Leech Seed) while with Encore the possibilities are endless (Using Substitute before for a status attack and then Encoring it, switching into Earthquakes and then Encoring it, etc), although PP is very tight with Encore and with switching negating its usefulness I personally lean more in favour of a flying attack.

EV Corner:

EVs: 252 Atk / 252 Spd / 6 SDef
Jolly Nature (+Spd, -SAtk)

Jumpluff doesn't need any HP since you want to be gaining more HP back then you spend on Subs. The less HP spent the cheaper the Subs are. Jumpluff's defences are near worthless when you consider that Jumpluff's Substitutes will be destroyed by anything that isn't NVE. If you choose to go with Encore then max out Special Defence and put whatever is leftover into Defence.

Other Optional Sets:

Jumpluff @ Leftovers or Liechi Berry
EVs: 6 HP / 252 Atk / 252 Spd
Jolly Nature (+Spd, -SAtk) - Swords Dance
- Sleep Powder
- Aerial Ace
- Hidden Power [Ground or Fighting] or Synthesis or Leech Seed or Substitute or Mimic

Sleep Powder something (it's especially useful to put to Sleep the Physical Wall or Hazer), use Sword Dance and then attempt to sweep. The choice of Hidden Power is optional. With Hidden Power [Fighting] you have neutral coverage on every Pokémon in the game while with Hidden Power [Ground] you have a 31 Speed IV for absolutely Max Speed. Synthesis or Leech Seed could be thrown on for some form of recovery, allowing the ability to set up on Pokémon who don't have proper offence (I.E Seismic Toss) while Substitute blocks statuses (and can allow the activation of a Liechi Berry). Due to Jumpluff's lack of move pool and decent number of physical resistances it could potentially get its hands on a good physical move while switching in (like Earthquake for example.).

Jumpluff @ Leftovers
EVs: 252 HP / 252 Spd / 6 SDef
Jolly Nature (+Spd, -SAtk)
- Swagger
- Sleep Powder
- Leech Seed
- Helping Hand

Sue me KKM! This is "Kari_Kagome_and_Mewtwo"s 2v2 Jumpluff (usually teamed with Aerodactyl, although anything faster than Jumpluff with good attack is a viable choice), where it uses Swagger on it's partner after they've set up Substitute (your partner will receive the attack boost but not the confusion). After that it gives support while its partner sweeps.

Strategy Against Jumpluff

Predicting Jumpluff switch ins hurts it badly (especially if you use Rock Slide when it thinks it's going into an EQ) and if Jumpluff's Sleep Powder misses, it usually leads to its demise. For versions which Seed-Stall, switching around and draining it of Leech Seed PP usually does the trick. Pokémon that are faster than Jumpluff usually have no trouble KOing it, although you'll rarely find yourself in a situation where you'll have a Subless Jumpluff against something faster than it.

Contest Moveset

A good Contest Moveset for Jumpluff would have to be this for the Smart Contest best with Calm, Gentle, Careful, Sassy Nature:

Attacks:

Toxic
Giga drain
Aerial ace
Hyper beam

Items Attached:

Green Scarf

Strategy Using Jumpluff

If you do the attacks in this following order you should have very few problems; 1st - Giga drain/Aerial ace, 2nd - Toxic, 3rd - Giga drain/Aerial ace, 4th - Toxic/Giga drain, 5th - Hyper beam

Locations in Games

Ruby/Sapphire/Emerald None, Must Trade from CFRLG Colosseum Evolve Skiploom Fire Red/Leaf Green Evolve Skiploom

Animé Appearences

Jumpluff has had a few Animé Appearences. First it was attacking Pokemon is Oak's Lab. After that Jenny used one to help solve a mystery. One was used in a Pokémon Cheerleading Squad & One lived with the Grass Pokemon in the Forbidden Forest Episode 227: Bulbasaur, The Ambassador! Episode 231: An Egg-Sighting Adventure! Episode 251: I Politoed You So! Episode 349: Grass Hysteria!